Photodynamic inactivation of recombinant bioluminescent Escherichia coli by cationic porphyrins under artificial and solar irradiation |
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Authors: | Eliana Alves Carla M B Carvalho João P C Tomé Maria A F Faustino Maria G P M S Neves Augusto C Tomé José A S Cavaleiro Ângela Cunha Sónia Mendo Adelaide Almeida |
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Institution: | (1) Department of Biology, CESAM, University of Aveiro, 3810-193 Aveiro, Portugal;(2) School of Health Technology, Porto Polytechnic Institute, 4200-465 Porto, Portugal;(3) Department of Chemistry, QOPNA, University of Aveiro, 3810-193 Aveiro, Portugal |
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Abstract: | A faster and simpler method to monitor the photoinactivation process of Escherichia coli involving the use of recombinant bioluminescent bacteria is described here. Escherichia coli cells were transformed with luxCDABE genes from the marine bioluminescent bacterium Vibrio fischeri and the recombinant bioluminescent indicator strain was used to assess, in real time, the effect of three cationic meso-substituted porphyrin derivatives on their metabolic activity, under artificial (40 W m−2) and solar irradiation (≈620 W m−2). The photoinactivation of bioluminescent E. coli is effective (>4 log bioluminescence decrease) with the three porphyrins used, the tricationic porphyrin Tri-Py+-Me-PF being the most efficient compound. The photoinactivation process is efficient both with solar and artificial light,
for the three porphyrins tested. The results show that bioluminescence analysis is an efficient and sensitive approach being,
in addition, more affordable, faster, cheaper and much less laborious than conventional methods. This approach can be used
as a screening method for bacterial photoinactivation studies in vitro and also for the monitoring of the efficiency of novel
photosensitizer molecules. As far as we know, this is the first study involving the use of bioluminescent bacteria to monitor
the antibacterial activity of porphyrins under environmental conditions. |
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Keywords: | Cationic porphyrins Photodynamic antimicrobial therapy Bioluminescence Escherichia coli Solar irradiation |
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