Large Specimen Bone Embedment and Cement Line Staining |
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Authors: | Helen E Gruber Audrey A Stasky |
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Institution: |
a Department of Orthopaedic Surgery, Carolinas Medical Center, Orthopaedic Research Biology, Charlotte, North Carolina |
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Abstract: | Undecalcified embedment of large bone specimens is often challenging. A method is presented here that is suitable for methacrylate embedment of sections of canine vertebrae while retaining the ability to localize tartrate-resistant acid phosphatase and alkaline phosphatase activity. Specimens also retained tetracycline labelling, and sectioned preparations were readily stained with routine bone procedures. A modification of the Bodian silver stain, used for examining the nerves and spinal cord in these specimens, provided a useful stain for canaliculi and cement lines in trabecular and cortical bone. This stain is advantageous when both bone and nerve tissue are of interest, as in spinal fusion studies. |
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Keywords: | bone histology cementlines enzyme histochemistry methacrylate |
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