Inactivation of bacteriophage k, Escherichia coli, and Candida albicans by ozone |
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| Authors: | I R Komanapalli B H S Lau |
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| Institution: | (1) Department of Microbiology and Molecular Genetics, School of Medicine, Loma Linda University, Loma Linda, California 92350, USA Tel.: +1 909 824 4480 Fax: +1 909 824 4035 e-mail: bLau@ccmail.llu.edu, US |
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| Abstract: | The effects of ozone (O3) on three types of microbes were studied. Test suspensions were exposed to 600 ppm O3 at room temperature. Control experiments were performed under identical conditions using oxygen gas. Bacteriophage λ was
completely inactivated at 10 min while Escherichia coli and Candida albicans were only inactivated by factors of 105 and 104 respectively at 40 min. Exposure of a mixed microbial suspension to O3 for 5 min resulted in 100% killing of bacteriophages while the viability of E. coli remained unchanged. Various body fluids containing phages were exposed to O3. Compared to buffered solution, the decrease in phage titers was significantly slower in whole blood, plasma, and albumin.
Both E. coli and C. albicans had increased production of thiobarbituric-acid-reactive substances with increased O3 exposure. 3H-labelled amino acids were incorporated into E. coli. O3 treatment resulted in a loss of radioactivity, indicating leakage of cytoplasmic contents. The data indicate that microbes
are inactivated by O3 at different rates, possibly related to differential membrane permeability. The milieu in which microbes are present determines
the effectiveness and outcome of O3 treatment.
Received: 15 October 1997 / Accepted: 24 February 1998 |
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