Construction of a recombinant human GM-CSF/MCAF fusion protein and study on its in vitro and in vivo antitumor effects |
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Authors: | YE Qinong SU Guofu ZHANG Shu HUANG Cuifen |
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Institution: | (1) Beijing Institute of Biotechnology, 100071 Beijing, China;(2) Department of Oncology, North Taiping Road Hospital, 100850 Beijing, China |
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Abstract: | A novel cytokine fusion protein was constructed by fusing granulocyte macrophage colony stimulating factor (GM-CSF) with monocyte
chemotactic activating factor (MCAF), which acts as a factor directing effector cells (monocytes) to a target site. The recombinant
human GM-CSF/MCAF fusion protein could sustain the growth of GMCSF-dependent cell line TF1 and was chemotactic for monocytes.
Thein vitro antitumor effect showed that rhGM-CSF/MCAF could activate monocytes to inhibit the growth of several human tumor cell lines,
including a promyelocyte leukemia cell line HL-60, a lung adenocarcinoma cell line A549, a hepatoma cell line SMMC-7721 and
a melanoma cell line Bowes. Furthermore, the cytotoxicity of monocytes activated by rhGM-CSF/MCAF against HL-60 and A549 was
greater than that activated by GM-CSF or MCAF alone, even greater than that activated by a combination of GM-CSF and MCAF,
suggesting that the fusion protein has synergistic or enhanced effects. Thein vivo antitumor effect indicated that rhGM-CSF/MCAF had marked antitumor effect against A549 tumor in nude mice and even completely
suppressed tumor formation. rhGM-CSF/MCAF was significantly more effective in inhibiting tumor growth than rhGM-CSF. Histological
analysis showed that tumor site injected with rhGM-CSF/MCAF was infiltrated by a large number of monocytes while a sparse
infiltration of monocytes was observed at the tumor site injected with rhGM-CSF or normal saline, suggesting that the antitumor
effect of rhGM-CSF/MCAF was mediated by the recruitment of a large number of monocytes to the tumor site. |
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Keywords: | GM-CSF MCAF gene cloning fusion protein antitumor |
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