Purification and Characterization of Two Types
of Chitosanase from a Microbacterium sp. |
| |
| Authors: | Yuying Sun Wanshun Liu Baoqin Han Jiquan Zhang Bing Liu |
| |
| Institution: | (1) College of Marine Life Sciences, Ocean University of China, Qingdao, 266003, P.R China;(2) Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, P.R China |
| |
| Abstract: | Two extracellular chitosanases (ChiX and ChiN) were extracted from Microbacterium sp. OU01 with Mr values of 81 kDa (ChiX) and 30 kDa (ChiN). ChiN was optimally active at pH 6.2 and 50°C and ChiX at pH 6.6 and 60°C (assayed over 15 min). Both the activities increased with the degree of deacetylation (DDA) of chitosan. ChiN hydrolyzed oligomers of glucosamine (GlcN) larger than chitopentaose, and chitosan with 62–100% DDA; but ChiX acted on chitosan and released GlcN. Hydrolysis of chitosan with 99% DDA by ChiN released chitobiose, chitotriose and chitotetraose as the major products. |
| |
| Keywords: | Chitooligosaccharides Chitosan Chitosanase Endo-type Exo-type Microbacterium sp |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
