Ssh AI restriction endonuclease from Salmonella shikmonah |
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| Authors: | Michiko Miyahara Katsuhisa Nakajima Tsutomu Kawanishi Katsutohi Mise |
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| Affiliation: | Division of Microbiology, National Institute of Hygienic Sciences, Tokyo, Japan. |
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| Abstract: | A new type II restriction endonuclease, SshAI, was purified from Salmonella shikmonah TK139 of kangaroo origin. The recognition and cleavage specificity of Ssh AI was determined to be 5'-CC/TNAGG-3', identical to that of SauI from Streptomyces aureofaciens and Bsu36I from Bacillus subtilis. Based on closely related and in part overlapping recognition specificities of Ssh AI and other restriction endonucleases, a close evolutionary relationship is proposed for all known Salmonella restriction endonucleases. |
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| Keywords: | Restriction endonucleases Salmonella Evolution |
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