Ginsenoside production,growth and cytogenetic characteristics of sustained <Emphasis Type="Italic">Panax japonicus</Emphasis> var. <Emphasis Type="Italic">Repens</Emphasis> cell suspension culture |
| |
Authors: | I N Smolenskaya O V Reshetnyak A V Nosov S E Zoriniants A L Chaiko Y N Smirnova A M Nosov |
| |
Institution: | (1) Department of Cell Biology and Biotechnology, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276 Moscow, Russia;(2) Institute of Food Chemistry and Technology, Ukrainian National Academy of Sciences and Ministry of Agricultural Politics, Osypovskogo 2a, 04123 Kyiv, Ukraine |
| |
Abstract: | Cytophysiological and cytogenetic characteristics of cell suspension culture of Panax japonicus var. repens were studied in relation to the accumulation of ginsenosides (GSs). The minimal time of cell number doubling was 1.3 ± 0.1
d and cell number increased 7 to 8-fold during growth cycle. The cell culture can be considered as aneuploid with about tetraploid
(46–60 chromosomes) modal class. Upon long-term cultivation, the total content of GSs considerably increased and maximal concentration
of GSs was 2.2 %(d.m.). The ratio of seven major GSs only slightly altered both over each and different subcultures. The overall
amount of GSs of Rg-group significantly exceeded that of Rb-group. Cell volume and the number of large cellular aggregates
with the higher proportion (by 20 %) of parenchymal cells increased late in the subculture. In this time the population contained
about 20 % of the cells with doubled amount of nuclear DNA and accompanied with elevation in the GS content. These data prompted
us to suggest that biosynthesis of GSs has a link with cell differentiation.
In memory of Prof. R.G. Butenko |
| |
Keywords: | Additional" target="_blank">Additional chromosome number cytodifferentiation ginseng in vitro culture nuclear DNA content triterpenes |
本文献已被 SpringerLink 等数据库收录! |