A rapid mini-prep method for isolation of ectomycorrhizal DNA from Tuber species期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

A rapid mini-prep method for isolation of ectomycorrhizal DNA from Tuber species

Authors:	Céline Di Battista Antonella Amicucci Chiara Guidi Luana Bertini Davide Sisti Vilberto Stocchi

Affiliation:	(1) Equipe de Microbiologie Forestière, Institut National de la Recherche Agronomique, Centre de la Recherches de Nancy, 54280 Champenoux, France;(2) Istituto di Chimica Biologica G. Fornaini', Università degli Studi di Urbino, v. Saffi, 2 – 61029 Urbino (PS), Italy;(3) Istituto Orto Botanico, Università degli Studi di Urbino, v. Bramante, 28 – 61029 Urbino (PS), Italy

Abstract:	A rapid procedure has been developed to isolate DNA from the ectomycorrhizae of Tuber spp. for use in PCR experiments. The method described is fast and sensitive and can overcome the amplification problems that can arise in the presence of inhibitors. For this reason it can be used to type ectomycorrhizae even starting from a single root tip and make mycorrhizae identification much more rapid.

Keywords:	DNA extraction ectomycorrhizae molecular identification Tuber
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