Enzyme activity in organic solvent as a function of water activity determined by membrane inlet mass spectrometry |
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| Authors: | Hans Degn David Lloyd |
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| Affiliation: | (1) Institute of Biochemistry, Odense University, DK-5230 Odense M, Denmark;(2) Sandor Bohatka, ATOMKI, H-4001 Debrecen Pf. 51, Hungary;(3) Microbiology Group, PABIO, University of Wales College of Cardiff, PO Box 915, CF1 3TL Cardiff, Wales, UK |
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| Abstract: | Summary Membrane inlet mass spectrometry (MIMS) is introduced as a method for measuring water activity in nonpolar solvents, aqueous solutions and gas phase. The determination of the rate of hydrolysis of diphenyl carbonate by porcine liver esterase as a function of water activity in diisopropyl ether is presented as an example. A linear relationship is found between the enzyme activity and the water activity. |
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