Isolation of mutants with altered pigments after irradiating haploid protoplasts from Datura innoxia Mill. with X-rays |
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Authors: | Otto Schieder |
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Affiliation: | (1) Abt. Straub, Max-Planck-Institut für Züchtungsforschung (Erwin-Baur-Institut), D-5000 Köln 30, Federal Republic of Germany |
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Abstract: | Summary Ten different mutants with altered pigment patterns were isolated following X-irradiation of approximately 105 haploid protoplasts of Datura innoxia Mill. Seven of the selected strains gave rise to shoots and 3 to leaves only. The mutants were selected from light green or white calli, which had developed 4 weeks after transfer of developing cell clusters onto B5 agar medium containing 0.5 mg/l BAP (Gamborg et al., 1968). Of the 10 mutant strains 5 were light green, two were yellow, one was pale yellow and one was white. One additional strain does not possess anthocyanin in its stems; a feature chracteristic of the wildtype is the possession of anthocyanin. This strain is able to grow in soil and has now flowered. None of the mutants obtained is haploid. Nine are diploid and the other is tetraploid. The chlorophyll deficient strains can be propagated on B5 agar medium supplemented with higher concentration of sucrose than normally required for the growth of the wild-type. |
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