| 舍蝇(Musca domestica vicina)凝集素的分离、纯化和部分性质研究 |
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| 引用本文： | 王霁,李根君,段吉群.舍蝇(Musca domestica vicina)凝集素的分离、纯化和部分性质研究[J].中国生物化学与分子生物学报,1991,7(1):47-53. |
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| 作者姓名： | 王霁 李根君 段吉群 |
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| 作者单位： | 山东大学生物系生化教研室，山东大学昆虫蛋白应用研究所，山东大学昆虫蛋白应用研究所 济南 250100，济南 250100，济南 250100 |
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| 摘 要： | 舍蝇蛹体液经抽提后上Sepharose 4B亲和层析柱纯化制得舍蝇凝集素。制剂经聚丙烯酰胺凝胶电泳和在SDS-PAGE上均呈单一蛋白带,表观分子量为33400。它能凝集人B型红细胞,亦能凝集小白鼠及兔血红细胞。其专一结合的糖为半乳糖与D-及L-岩藻糖。
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| 关 键 词： | 舍蝇 凝集素 |
| 收稿时间： | 1991-02-20 |
Studies on the Isolation, Purification and Some Properties of Lectin from Musca domestica vicina |
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| Wang,Ji Li,Gen-jun Duan,Ji-qun.Studies on the Isolation, Purification and Some Properties of Lectin from Musca domestica vicina[J].Chinese Journal of Biochemistry and Molecular Biology,1991,7(1):47-53. |
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| Authors: | Wang Ji Li Gen-jun Duan Ji-qun |
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| Institution: | (Department of Biology, Shandong University, Jinan 250100 |
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| Abstract: | A lectin was purified from pupa of Musca demestica vicina by Sepha-rose 4B affinity chromatography. This lectin showed one band on polyacrylamide gel electrophoresis. The apparent molecular weight of it, as determined by SDS-PAGE, is 33400. This lectin agglatinated human B-type red blood cells, rat and rabbit red blood cells. The hemagglutination activity was inhibited by galactose and fucose. |
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| Keywords: | Musca demestica vicina Lectin |
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