| 我国海南省杀蚊球形芽孢杆菌的分离和分布 |
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| 引用本文： | 袁志明, 陈宗胜, 刘娥英, 蔡全信, 张用梅,. 我国海南省杀蚊球形芽孢杆菌的分离和分布[J]. 微生物学通报, 1997, 24(4): 203-205 |
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| 作者姓名： | 袁志明 陈宗胜 刘娥英 蔡全信 张用梅 |
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| 作者单位： | 中国科学院武汉病毒研究所; |
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| 基金项目： | 国家自然科学基金，中国科学院微生物所做生物资源前期开发实验室资助 |
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| 摘 要： | 在我国海南省共采集158个各种类型的泥土样品，采用选择性培养基（PBMYS）进行样品分离，并结合形态学观察、血清学鉴定和生物测定，获得对致倦库蚊（Culexquinquefasciatus）幼虫有毒性的球形芽孢杆菌（Bacillussphaericus）73株，有毒株的分离率占样品总数的342％。血清学试验表明，新分离菌株有71株属H5a5b型，两株属在我国首次分离出比型菌株。许多球形芽bqn杆菌新菌株对致倦库蚊幼虫具有很强的毒杀作用。其中228－2和117－1菌株的丙酮粉剂效
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| 关 键 词： | 球形芽孢杆菌 致倦库蚊 效价 血清型 |
ISOLATION OF MOSQUITO-LARVICIDAL BACILLUS SPHAERICUS FROM THE SOIL SAMPLES COLLECTED IN HAINAN, CHINA |
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| Yuan Zhiming Chen Zongsheng Liu Eying Cai Quanxing Zhang Yongmei. ISOLATION OF MOSQUITO-LARVICIDAL BACILLUS SPHAERICUS FROM THE SOIL SAMPLES COLLECTED IN HAINAN, CHINA[J]. Microbiology China, 1997, 24(4): 203-205 |
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| Authors: | Yuan Zhiming Chen Zongsheng Liu Eying Cai Quanxing Zhang Yongmei |
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| Abstract: | During 1992-1993, one hundred and fifty eight of vanous soil samples collected in fourteen counties or cities in Hainan Province. With a selected medium (PBMYS), totally seventy-three strains of Bacillus sphaericus, possessing larvicidal achvity against Culex quinquefasciatus, were isolated. The rate of active strains isolated is 34.2% in the total of samples. The seroIogical study showed that most of newly isolates were belonging to serotype Wash except that tWo strains, 47-6b and 124-3, were belonging to H6.The acetone powders prepared from several newly isolates of H5aa5b, were highiy toxic to the larvae of C quinquefasciatus. Two of them, 228-2 and 117-1, had a calculated Potency of 2721 and 2331ITU/ mg respechvely. In contraSt with the potent sthen 2362, it had a potency value of 2218ITU/mg. |
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| Keywords: | Bacillus sphaericus Culex quinquefasciatus Potency Serotype |
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