| 枯草杆菌BS-98分泌的抗真菌蛋白的分离纯化及其部分性质的研究 |
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| 引用本文： | 胡剑, 赵永歧, 王岳五,.枯草杆菌BS-98分泌的抗真菌蛋白的分离纯化及其部分性质的研究[J].微生物学通报,1997,24(1):3-7. |
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| 作者姓名： | 胡剑 赵永歧 王岳五 |
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| 作者单位： | 南开大学生命科学学院微生物系; |
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| 摘 要： | 拮抗菌（Bacillussubtilis）BS-98菌株在BPY液体培养基中产生的蛋白质经硫酸铵分级盐析、SephsdexG-100柱层析、DEAE-纤维素柱层析和SephadexG－100柱再层析后，分离纯化得到的抗菌蛋白在电泳中显现出三条带。经初步纯化的抗菌蛋白对热稳定，对蛋白酶部分敏感。抑菌谱测定表明，抗菌蛋白对芦笋茎枯病菌、小麦赤霉病菌、棉花枯萎病菌、棉花黄萎病菌、灰霉菌、立枯丝核菌等病原真菌及黄瓜角斑病菌都具有强烈的抑制作用。
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| 关 键 词： | 拮抗菌 抗真菌蛋白质 纯化 抑菌活性 芦笋茎枯病菌 |
PURIFICATION AND SOME PROPERTIES OF ANTIFUNGAL PROTEIN SECRETED FROM ANTAGONISTIC BACILLUS SUBTIL IS BS-98 |
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| Hu Jian,Zhao Yongqi,Wang Yuewu.PURIFICATION AND SOME PROPERTIES OF ANTIFUNGAL PROTEIN SECRETED FROM ANTAGONISTIC BACILLUS SUBTIL IS BS-98[J].Microbiology,1997,24(1):3-7. |
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| Authors: | Hu Jian Zhao Yongqi Wang Yuewu |
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| Abstract: | The antifungal protein purified by ammonium sulphate precipitation and columnchromatography on Sephadex G-100, and DEAE-Cellulose was demonstrated to be of threeprotein bands by polyacrylamide gel electrophoresis The protein was found to be thermostableand partially sensitive to proteinases. The inhibitory spectum showed that the protein had astrong inhibiting activity against the pathogens of Phoma asparagi,, Fusarium graminearum,Fusarium oxysporum f. sp. vasinfectum, Verticillium, albo-atrum, Botrytis cinerea, Rhizoctoniasolani, Pseudomonas Syringae pv. lachrmans. |
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| Keywords: | Antagonstic bacterium Antifungal protein Purification Inhibiting activity Phoma asparagi Sacc |
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