检测结直肠组织中人乳头瘤病毒DNA的研究

用人乳头瘤病毒（HPV）高度保守的通用引物和16、18型特异引物对123例给直肠组织作聚合酶链反应（PCR）检测HPVDNA，总检出率为14．60％正常粘膜、结肠炎和炎性息肉组为3．3％（2／71），乳头状腺瘤为17．6％（3／17），原位癌和浸润癌为37．1％（13／35）．在正常组织、癌旁组织和癌组织HPVDNA阳性率分别为2．9％、5．7％和37.1％．在结直肠腺癌中HPV感染以18型多见，18型与16型之比为3:1，HPV在左半结肠以下比右半结肠感染率高。

DELECTION OF HUMAN PAPILLOMAVIRUS DNA IN COLORECTAL CARCINOMA

Piopsy spesmens specimens from colorectum of 123 pahents were analysised for human papiIlomavirus (HPV) by polymerase chain reachon (PCR). We used genera consensus primers and type-specific primers (16,18). HPV DNA was delected in 14.6% of Piopies-There were two of 17 in normal (3.3%), colorechhs and inflammatory polyps, three of 17 in papillate adenomas (17.6%) and thirteen of 35 in colorectal. Detection rate of HPV DNA in normal, adjacent carcinoma and carcinoma were 13 / 35 (37.1%), 2 / 35(5.7%) and 1 / 35(2.9%)respechvely. HPV was predominahy type 18 in colorectal carcinoma. The inflammatory rate is higher in rectum and left colon than right colon.