尼泊尔马桑根瘤内生菌纯培养物的质粒检测

采用胶内裂解法快速检测了21株马桑根瘤内生菌纯培养物和4株弗兰克氏菌参考菌株的质粒，其中有5株马桑分离菌株和1株参考菌株含有质粒。除马桑菌株和参考菌株各有1株携带2个质粒外，其它菌株均只含有1个质粒。这些质粒的分子量约为13～20kb。根据所含质粒的大小和数目，将21株马桑分离菌株划分成4个质粒类群。实验还对菌丝体生长，细胞酶解和裂解等条件对质粒检测效果的影响进行了探讨。

PLASMID DETECTION FOR PURE CULTURES OF NODULAR ENDOPHYTES OF CORIARIA NEPALENSIS

A rapid ingel lysis method was used to detect the plasmids among twenty one Frankia strains from Coriaria nepalensis and four confirmed Frankia strains from other host plants belonging to four genera of actinorhizal trees. Five isolates were detected harbouring plasmids which ranged from 13 to 20 kb in size. Both the Frankia strain Hr18 and the isolate Cs466 showed two plasmids, They appered to share the same plasbod pattern according to the size. Based on the number and size of the plasmids detected, twenty one Coriaria isolates were divided into four plasmid groups. The effect of bacterial growth and cell lysis conditions on plasmid detection was also explored.