| 曲酸菌选育及发酵工艺研究 |
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| 引用本文： | 孙微, 陶文沂,.曲酸菌选育及发酵工艺研究[J].微生物学通报,1997,24(5):274-277. |
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| 作者姓名： | 孙微 陶文沂 |
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| 作者单位： | 无锡轻工大学生物工程学院; |
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| 摘 要： | 筛选获得产曲酸菌株米曲霉（Aspergillusoryzae）MSA进行60Co诱变，并进行了发酵工艺条件研究，以葡萄糖为碳源、豆饼粉为氮源，在pH3.0、33℃摇瓶培养，可达到5d产酸5％以上水平。发酵液采用直接浓缩结晶工艺，脱色与重结晶后获得无色针状晶体，红外图谱检测确证为曲酸。
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| 关 键 词： | 曲酸 米曲霉 60Co诱变 |
STUDIES ON THE BREEDING AND FERMENTATION CONDITIONS OF KOJIC ACID PRODUCING STRAIN |
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| Sun Wei,Tao Wenyi.STUDIES ON THE BREEDING AND FERMENTATION CONDITIONS OF KOJIC ACID PRODUCING STRAIN[J].Microbiology,1997,24(5):274-277. |
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| Authors: | Sun Wei Tao Wenyi |
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| Abstract: | ASP. oryzae MSA was screened from 12 strains. Two mutants Chllh and AC6 with higher ablity of production of kojic acid were obtained by UV and 60Co ray. Fermentation technology was studied, the result showed that the two stains can produce kojic acid over 5% after five days. Fermentation broth was concentrated directly, decolorized and recryshtized, and the product of prismatic needles were obtained. |
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| Keywords: | Kojic acid Asp oryzae Mutanntion |
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