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Electrophoretic karyotype of Saprolegnia monoica
Authors:Maryline Mort-Bontemps  Michel Fèvre
Institution:Laboratoire de Biologie Cellulaire Fongique, Centre de Génétique Moléculaire et Cellulaire, UMR CNRS 106, UniversitéLyon 1, 43 boulevard du 11 novembre 1918, 69622 Villeurbanne Cedex, France
Abstract:Abstract The electrophoretic karyotype of Saprolegnia monoica was determined by contour-clamped homogeneous electric field (CHEF) gel electrophoresis. Eight chromosomal bands were separated. The size of these bands, based on migration relative to those of chromosomal DNA of Saccharomyces cerevisiae , Schizosaccharomyces pombe and Hansenula wingei , is estimated to be between 0.9 and 5.8 Mb. The genome size is estimated to be 51 Mb.
Keywords:Saprolegnia monoica            Chromosomes  Electrophoretic karyotype  Contour-clamped homogeneous electric field (CHEF) gel electrophoresis
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