Establishment of stable human fibroblast cell lines constitutively expressing active Rho-GTPases |
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Authors: | S Servotte Z Zhang C A Lambert T T Giang Ho G Chometon B Eckes T Krieg C M Lapière B V Nusgens M Aumailley |
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Institution: | (1) Laboratory of Connective Tissues Biology, Centre de Recherche de la Groupement Interdisciplinaire de Génomique Appliquée, University of Liège, Liège;(2) Center for Biochemistry, University of Cologne, Cologne;(3) Center for Molecular Medicine, University of Cologne, Cologne;(4) Department of Dermatology, University of Cologne, Cologne |
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Abstract: | Summary. Small GTP-binding proteins of the Rho family (RhoA, Cdc42, Rac1) regulate the organisation and the turnover of the cell’s
cytoskeleton and adhesion structures. A significant function of these cellular structures is to translate and counterbalance
forces applied to, or generated by, cells in order to maintain homeostasis and control cell movement. We therefore hypothesised
that Rho-GTPases are directly involved in cellular gravity perception and may participate in the alterations induced in microgravity.
To define an adequate cellular model allowing to investigate this issue, we have established stable cell lines constitutively
expressing active forms of either RhoA, Cdc42, or Rac1. The three cell lines differ by morphology and by their ability to
form filopodia, lamellipodia, and bundles of actin stress fibers. Overexpression of the active form of either RhoA, Cdc42,
or Rac1 is compatible with cell viability and does not affect cell population doubling time. Thus, our series of mutant cells
appear well suited to gain further knowledge on the molecular mechanisms of cellular gravity perception.
Correspondence and reprints: Institute for Biochemistry II, Joseph-Stelzmann-Strasse 52, 50931 Cologne, Federal Republic of
Germany. |
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Keywords: | : Fibroblast GTP-binding protein of Rho family Cytoskeleton Proliferation |
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