Investigations into the occurrence of alkaloids in ergot and single sclerotia from the 2007 and 2008 harvests |
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Authors: | M Appelt F M Ellner |
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Institution: | 1.Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Ecochemistry, Plant Analysis and Stored Product Protection,Berlin,Germany |
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Abstract: | As a contribution to the occurrence of ergot alkaloids in ergot from German rye and triticale, samples from the 2007 and 2008
harvests were analyzed. Twelve alkaloids—six pairs of main alkaloids and their corresponding epimers—were determined in extracts
prepared under alkaline conditions by HPLC with fluorescence detection without preceding purification. The total alkaloid
content was found to be 0.03–0.18% in ergot from rye (n = 19) and 0.06–0.22% in ergot from triticale (n = 4), respectively. Furthermore, single sclerotia (n = 40) were investigated in terms of alkaloid content and distributional pattern. The main alkaloids in ergot were ergocristine,
ergotamine and ergocornine, although the alkaloid composition was highly variable.
Presented in part at the 30th Mykotoxin-Workshop, Utrecht, The Netherlands, April 28–30, 2008 |
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Keywords: | Ergot Ergot alkaloids Rye Triticale Regulation HPLC-FLD |
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