Fluorescence polarization of trypsin digested photosystem II membranes |
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Authors: | Ranjana Paliwal G S Singhal |
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Institution: | (1) School of Life Sciences, Jawaharlal Nehru University, 110067 New Delhi, India |
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Abstract: | Fluorescence polarization of photosystem II particles treated with trypsin and incubated with high salt-medium (2M NaCl) was investigated. The presence of atrazine and TMPD in normal and salt-washed particles induced a decrease in the polarization ratios. Similar results were obtained at low concentrations of trypsin. On the basis of our observations we suggest that the presence of these perturbing agents causes a reorganisation of the membrane components and alters pigment-pigment and pigment-protein interactions. The results of fluorescence polarization demonstrate trypsin entry into the membrane after the digestion of the peripheral proteins.Abbreviations Chl
chlorophyll
- Mes
2-(N-morpholino)-ethanesulfonic acid
- OEC
oxygen evolving complex
- PS II
photosystem II
- TMPD
N, N, N
- N
tetramethyl-p-phenyl-enediamine
- DPH
1, 6-diphenyl-1, 3, 5-hexatriene |
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Keywords: | fluorescence polarization membrane organisation photosystem II salt-washing trypsin digestion |
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