Cre recombinase specificity defined by the tau locus |
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Authors: | Korets-Smith Ella Lindemann Lothar Tucker Kerry Lee Jiang Caoyang Kabacs Nikolett Belteki Gusztav Haigh Jody Gertsenstein Marina Nagy Andras |
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Institution: | Samuel Lunenfeld Research Institute, 600 University Ave., Toronto, Ontario, M5G 1X5, Canada. |
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Abstract: | We generated a transgenic mouse line (tau::Cre) by targeting the Cre to the tau locus (Mapt). Based on previous reports on the expression of Tau during development, we expected the Cre recombinase to be expressed in a neuron-specific and pan-neuronal manner. However, intercrosses between the tau::Cre and the Cre-activatable reporter animals resulted in offspring with recombination either restricted to the nervous system or throughout the entire conceptus, indicating expression of Tau early in development. The percentage of neuron-specific excision was dependent on the Cre reporter used representing different Cre target sites in the mouse genome. In spite of the observed variability, our data suggest that the tau::Cre mouse line can be used for pan-neuronal recombination of floxed alleles when it is used with caution. |
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Keywords: | Cre/loxP tau neuron‐specific Z/EG Cre reporters |
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