In situ and in vitro colonization of Cathaya argyrophylla (Pinaceae) by ectomycorrhizal fungi |
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Authors: | Lu-Min Vaario Shu-Tang Xing Zong-Qiang Xie Zhi-Ming Lun Xue Sun Yu Hua Li |
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Institution: | (1) Research Institute of Flower Biotechnology, Northeast Forestry University, 150040 Harbin, China;(2) The Laboratory of Quantitative Vegetation Ecology, The Institute of Botany, CAS, 100093 Beijing, China |
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Abstract: | Cathaya argyrophylla, a critically endangered conifer, is found to grow at four isolated areas located in subtropical mountains of China. To examine
the involvement and usefulness of mycorrhizas for sustaining the population of this tree, we compared the root system, morphology,
and structure of mycorrhizal roots of C. argyrophylla, which were collected from a natural stand and an artificial stand, each grown at a different location. More mycorrhizal
roots were found for trees from an artificial stand. The presence of extramatrical mycelium, mantle, and Hartig net revealed
that C. argyrophylla formed an ectomycorrhizal association in both sampling sites. Starch granules were found in mycorrhizal roots collected only
from a natural stand. The aseptic synthesis of C. argyrophylla and Cenococcum geophilum was established for the first time in vitro. Typical ectomycorrhizas formed on seedlings on RM medium containing 0.1 g/l
glucose, 5 weeks after inoculation. By light microscopy, the synthesized mycorrhizas showed a thin mantle from which emanated
extramatrical hyphae and highly branched Hartig net. A simple, rapid, and convenient mycorrhiza synthesis system was developed,
which facilitates further studies on ectomycorrhizal development of C. argyrophylla. |
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Keywords: | Cathaya argyrophylla Ectomycorrhizal fungi In vitro In situ Hartig net |
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