In vitro stimulation of lecithin synthesis in rat liver mitochondria and microsomes after treatment with phospholipase C

Rat liver mitochondria in which diglycerides were generated by phospholipase C treatment were shown to incorporate labeled choline from cytidine-5′diphospho-Me-¹⁴C] choline into lecithin to an extent which could not be ascribed to microsomal contamination. The response of this enzymatic activity to the extent of phospholipase C degradation was qualitatively different in microsomes and mitochondria, suggesting clearly different properties of this enzyme in the two subcellular fractions.