Antitumour activity of Salmonella typhimurium VNP20047 in B16(F10) murine melanoma model |
| |
Authors: | Jazowiecka-Rakus Joanna Szala Stanisław |
| |
Institution: | Department of Molecular Biology, Centre of Oncology, Maria Sk?odowska-CurieMemorial Institute, Gliwice, Poland. jjazowiecka@io.gliwice.pl |
| |
Abstract: | A tumour therapy is proposed based on attenuated Salmonella typhimurium VNP20047 expressing the Escherichia coli cytosine deaminase gene. VNP20047 was administered intravenously to B16(F10) melanoma-bearing C57BL/6 mice. VNP20047 proliferated within tumours and livers regardless of the initial inoculum dose. After 10 days the number of bacteria increased in livers up to 4.2 x 10(6) cfu/g and decreased in tumours down to 5.9 x 10(6) cfu/g. VNP20047 at 1 x 10(5) cfu/mouse, when combined with 5-fluorocytosine, inhibited tumour growth by 85% without prolonging animal survival. Histology studies revealed severe lesions in tumours and livers. These data suggest that S. typhimurium VNP20047 induced inflammatory responses, even though the strain was attenuated. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|