In vivo growth conditions suppress the expression of ganglioside GM2 and favour that of lacto series gangliosides in the human glioma D-54MG cell line |
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Authors: | Pam Fredman Carol J Wikstrand Jan-Eric Månsson Guido Reifenberger Sandy H Bigner Ahmed Rasheed Lars Svennerholm Darell D Bigner |
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Institution: | (1) Institute of Clinical Neuroscience, Department of Psychiatry and Neurochemistry, Göteborg University, Mölndal Hospital, S-431 80, Sweden;(2) Department of Pathology and Preuss Laboratory for Brain Tumor Research, Duke University Medical Center, Box 3156, 27710 Durham, NC, USA;(3) Department of Neuropathology, Heinrich-Heine University, Box 101007, D-40001 Düsseldorf, Germany |
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Abstract: | The human glioma D-54MG cell line grownin vitro primarily expresses ganglio series gangliosides, particularly GM2. Subcutaneous injection of these cells into nude mice produced xenografts with an increased content of the human glioma-associated lacto series gangliosides, primarily 3 -isoLM1, an alteration that was dose dependent, with the highest dose (1×108) resulting in a phenotype that was most like that of the inoculum. After one passagein vivo, the lacto series dominated and reached a proportional level that was kept throughout the 10 passages. The mRNA levels of the GM2-synthase clearly coincided with GM2 expression and was 20 times higher in cells grownin vitro than in those grownin vivo. These results support the view that ganglioside expression in human gliomas is strongly influenced by environmental factors.
Abbreviations: The gangliosides have been designated according to Svennerholm (Eur J Biochem (1977)79: 11–21) GM3, II3NeuAc-LacCer; GM2, II3NeuAc-GgOse3Cer; GM1, II3NeuAc-GgOse4Cer; GD3, II3(NeuAc)2-LacCer; GD2, II3(NeuAc)2-GgOse3Cer; GD1a, IV3NeuAc, II3NeuAc-GgOse4Cer; GD1b, II3(NeuAc)2-GgOse4Cer; GT1b, IV3NeuAc, II3(NeuAc)2-GgOse4Cer; 3 -LM1, IV3NeuAc-nLcOse4Cer; 3 -isoLM1, IV3NeuAc-LcOse4Cer; 3 ,6 -isoLD1, IV3NeuAc, III6NeuAc-LcOse4Cer; 3 8 -LM1, IV3(NeuAc)2-nLcOse4Cer. MAb(s), monoclonal antibody (ies); the designation LM1 is used when both 3 -isoLM1 and 3 -LM1 and LD1, when both 3 6 -isoLD1 and 3 8 -LD1 are included. |
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Keywords: | Ganglioside antigens gliomas ganglioside metabolism glioma cell line |
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