Application of enzymatic apple pomace hydrolysate to production of 2,3-butanediol by alkaliphilic <Emphasis Type="Italic">Bacillus licheniformis</Emphasis> NCIMB 8059 |
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Authors: | Aneta M Bia?kowska Ewa Gromek Joanna Krysiak Barbara Sikora Halina Kalinowska Marzena J?drzejczak-Krzepkowska Celina Kubik Siegmund Lang Fokko Schütt Marianna Turkiewicz |
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Institution: | 1.Institute of Technical Biochemistry,Lodz University of Technology,Lodz,Poland;2.Institute of Biochemistry and Biotechnology,Technical University Braunschweig,Brunswick,Germany;3.Thünen Institute of Wood Research,Hamburg,Germany |
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Abstract: | 2,3-Butanediol (2,3-BD) synthesis by a nonpathogenic bacterium Bacillus licheniformis NCIMB 8059 from enzymatic hydrolysate of depectinized apple pomace and its blend with glucose was studied. In shake flasks, the maximum diol concentration in fed-batch fermentations was 113 g/L (in 163 h, from the hydrolysate, feedings with glucose) while in batch processes it was around 27 g/L (in 32 h, from the hydrolysate and glucose blend). Fed-batch fermentations in the 0.75 and 30 L fermenters yielded 87.71 g/L 2,3-BD in 160 h, and 72.39 g/L 2,3-BD in 94 h, respectively (from the hydrolysate and glucose blend, feedings with glucose). The hydrolysate of apple pomace, which was for the first time used for microbial 2,3-BD production is not only a source of sugars but also essential minerals. |
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