| 冰晶核蛋白及其在细菌表面展示技术中的应用 |
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| 作者单位： | ;1.浙江工业大学药学院 |
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| 摘 要： | 冰晶核蛋白(ice nucleation protein,INP)是一种分泌型外膜蛋白,广泛分布于丁香假单胞菌,荧光假单胞菌和其他革兰氏阴性菌中。由于其在相对高温下(-2~-4℃)形成冰核的特性,INP最早应用于生物制冷领域。在细菌表面展示技术中,冰晶核蛋白作为运载蛋白得到广泛的应用。与其他的表面技术载体蛋白相比较,冰晶核蛋白具有稳定表达外源蛋白及展示分子量较大的外源蛋白的优点。INP细胞表面展示技术已被应用于全细胞生物催化剂、全细胞吸附剂和环境污染物降解剂等的开发,本文将简述INP表面展示技术的研究进展。
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| 关 键 词： | 冰晶核蛋白(INP) 表面展示 载体蛋白 |
Ice Nucleation Protein and Its Applications in Bacterial Cell-Surface Display Technology |
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| Institution: | ,College of Pharmaceutical Science,Zhejiang University of Technology |
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| Abstract: | Ice nucleation protein(INP)is a secretory outer membrane protein from Pseudomomas syringae,P.flurorescens and several other Gram-negative bacteria.Using its characteristic of forming ice nucleation at relatively warm temperatures(-2~-4 ℃),INP is originally applied in biological refrigeration field.In the bacterial surface display technology,INP is widely used as a functional carrier protein.Compared with other carrier proteins,INP has advantages such as stably expressing foreign proteins and showing the larger molecular weight proteins.INP cell-surface display technology has been developed to be a promising approach in many biotechnological fields,such as whole cell bioeatalysts,whole cell bioadsorbence and biodegradation of environmental pollutants.This article reviews the recent research advances in INP cell-surface display. |
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| Keywords: | ice nucleation protein(INP) surface display carrier protein |
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