Hairy root culture of Plumbago indica as a potential source for plumbagin |
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Authors: | M. Gangopadhyay D. Sircar A. Mitra S. Bhattacharya |
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Affiliation: | (1) Medicinal Plant Laboratory, Department of Botany, Bose Institute, 93/1 A.P.C. Road, Kolkata, 700009, India;(2) Agricultural and Food Engineering Department, Indian Institute of Technology Kharagpur, Kharagpur, 721302, India |
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Abstract: | Hairy roots of Plumbago indica were established at high frequency (90 %) by infecting leaf explants with Agrobacterium rhizogenes strain ATCC 15834. The axenic root cultures were established under darkness in hormone-free liquid Murashige and Skoog medium containing 3 % sucrose. The highest plumbagin content was found to accumulate in roots at their exponential phase of growth. A low pH (4.6) and a low concentration of sucrose (1 %) were beneficial for root growth in darkness, while pH 5.6 and 3 % sucrose under continuous irradiance enhanced plumbagin accumulation in roots up to 7.8 mg g−1(d.m.). Direct shoot regeneration from hairy root culture was also achieved under continuous irradiance, thus indicated an easy way of obtaining transformed P. indica plants. |
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Keywords: | KeywordHeading" >Additional Agrobacterium rhizogenes transformation organogenesis |
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