Tissue-specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants |
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Authors: | Oscar Vorst Frans van Dam Renske Oosterhoff-Teertstra Sjef Smeekens Peter Weisbeek |
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Affiliation: | (1) Department of Molecular Cell Biology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, Netherlands;(2) Institute of Molecular Biology, University of Utrecht, Padualaan 8, 3584 CH Utrecht, Netherlands |
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Abstract: | We have isolated and analyzed a pre-ferredoxin gene from Arabidopsis thaliana. This gene encodes a 148 amino acid precursor protein including a chloroplast transit peptide of 52 residues. Southern analysis shows the presence of a single copy of this ferredoxin (Fd) gene in the A. thaliana genome. Its expression is tissue-specific and positively affected by light. Response times, both to dark and light conditions, are remarkably rapid.A chimeric gene consisting of a 1.2 kb Fd promoter fragment fused to the -glucuronidase reporter gene was transferred to tobacco. This fusion gene is expressed in a tissue-specific way; it shows high levels of expression in green leaves, as compared to root tissue. |
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Keywords: | Arabidopsis thaliana pre-ferredoxin /content/m400t368t701k0k7/xxlarge946.gif" alt=" beta" align=" MIDDLE" BORDER=" 0" >-glucuronidase reporter gene light-regulated expression transgenic tobacco |
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