Variation of wheatgrass chromosomes in wheat-wheatgrass alien addition line "TAI-27"revealed by fluorescence in situ hybridization (FISH) |
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Authors: | HAN FangpuZHANG Xiangqi BU XiulingHE MengyuanHAO ShuiMA Youzhi XIN Zhiyong |
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Institution: | (1) Institute of Genetics and CytoIogy, Northeast Normal University, 130024 Changchun, China;(2) Institute of Crop Breeding and Cultivation, Chinese Academy of Agriculture Sciences, Crop Genetics and Breeding, Ministry of Agriculture Key Laboratory, 100081 Beijing, China |
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Abstract: | The Karyotyp of the primary wheat-whastgrass alien addition line TAI-27 was 2n = 44 in which all d the chromosomes were metacentric
and subrmetacentric. However, in the progeny of TAI-27 a pair of chromosomes had become small chromosomea in the two morphologically
different plants. Fluorescence in situ hybridizstionm (FISH) technique was used to analyze the two different plants. The observations
indicate that a pair of small chromosomes in one varietion line are from wheatgrass. In another variation line, a pair of
small chromosomes are also from whest-grass, while another pair of wheatgrass chromosomes have substituted the wheat chromosomes.
TAI-27 and its variant lines showed a high level of resistance to barley yellow dwarf virus (BYDV). The pessible explanation
for such a variation and the potential use of the variant lines were discussed briefly. |
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Keywords: | wheat wheatgrass alien additlon line alien addition-subatitutlon line fluorscencein situ hybridization (FISH) |
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