The protective efficacy of chlamydial protease-like activity factor vaccination is dependent upon CD4+ T cells |
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Authors: | Murphey Cathi Murthy Ashlesh K Meier Patricia A Neal Guentzel M Zhong Guangming Arulanandam Bernard P |
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Institution: | Department of Biology, University of Texas at San Antonio, San Antonio, TX 78249, USA. |
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Abstract: | We have previously determined the protective efficacy of intranasal vaccination with chlamydial protease-like activity factor (CPAF) against genital chlamydial infection. Since T-helper 1 (Th1) responses are important for anti-chlamydial immunity, we examined the contribution of CD4(+) T cells in CPAF mediated immunity against intravaginal (i.vag.) Chlamydia muridarum infection in C57BL/6 mice. CPAF+IL-12 vaccination induced antigen-specific CD4(+) T cells that secreted elevated levels of IFN-gamma, and generated strong humoral responses. The protective effects of CPAF vaccination against genital chlamydial challenge were abrogated by anti-CD4 neutralizing antibody treatment. Moreover, anti-chlamydial immunity could be adoptively transferred to na?ve recipients using CPAF-specific CD4(+) T cells. Therefore, CPAF mediated anti-chlamydial immunity is highly dependent upon antigen-specific CD4(+) T cells. |
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Keywords: | Chlamydia Vaccine Chlamydial protease-like activity factor CD4+ T cells Th1 |
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