Genetic studies of the ribosomal proteins in Escherichia. coli. II. Altered 30s ribosomal protein component specific to spectinomycin resistant mutants |
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| Authors: | Satoshi Dekio and Renkichi Takata |
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| Institution: | (1) Department of Biochemistry and Biophysics, Research Institute for Nuclear Medicine and Biology, Hiroshima University, Kasumi-cho, Hiroshima, Japan |
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| Abstract: | Summary Spectinomycin resistant (spc
r) mutants were obtained by treating the cells of E. coli K12, W3637 with nitrosoguanidine. The compositions of ribosomal proteins were analyzed for six out of eleven such spc
r-mutants with chromatography on a carboxymethyl cellulose (CMC) column. The 30s ribosomal subunit from all of the spc
r-mutants was found to contain the altered 30-4 protein component, while no difference was detected in 50s ribosomal proteins between spc
r and spc
s bacteria.Abbreviations used CMC
carboxymethyl cellulose
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str
streptomycin
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spc
spectinomycin |
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| Keywords: | |
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