Studies on the biochemical basis of mutation VI. Selection and characterization of a new bacteriophage T4 mutator DNA polymerase |
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Authors: | L J Reha-Krantz M J Bessman |
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Affiliation: | The McCollum-Pratt Institute and The Department of Biology The Johns Hopkins University, Baltimore, MD 21218, U.S.A. |
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Abstract: | A new mutant of bacteriophage T4 has been isolated by a procedure which was designed to select for mutants with high spontaneous reversion rates. This mutant, M19, induces a defective DNA polymerase which has a degraded specificity and makes errors by inserting the incorrect nucleotide more frequently than the wild-type enzyme.In addition to M19, several other T4 polymerase amber and temperature-sensitive mutants have been located on a linear, fine-scale map. The mutants which most strongly affect mutation rates are found in two clusters at 25% and 80% of the gene. These two domains may represent the active site(s) of the polymerase and exonuclease activities. |
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