Zirex: a Novel Zinc-Regulated Expression System for Lactococcus lactis |
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| Authors: | Dongdong Mu Manuel Montalbán-López Yoshimitsu Masuda Oscar P. Kuipers |
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| Affiliation: | Department of Molecular Genetics, University of Groningen, Linnaeusborg, Groningen, The Netherlandsa;The Kluyver Centre for Genomics of Industrial Fermentation, Groningen-Delft, The Netherlandsb |
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| Abstract: | Here, we report a new zinc-inducible expression system for Lactococcus lactis, called Zirex, consisting of the pneumococcal repressor SczA and PczcD. PczcD tightly regulates the expression of green fluorescent protein in L. lactis. We show the applicability of Zirex together with the nisin-controlled expression system, enabling simultaneous but independent regulation of different genes. |
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