Foamy Virus Gag p71-p68 Cleavage Is Required for Template Switch of the Reverse Transcriptase |
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| Authors: | Ralf Spannaus Anna Schneider Maximilian J. Hartl Birgitta M. W?hrl Jochen Bodem |
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| Affiliation: | Universität Würzburg, Institut für Virologie und Immunbiologie, Würzburg, Germanya;Universität Bayreuth, Lehrstuhl für Struktur und Chemie der Biopolymere, and Research Center for Biomacromolecules, Bayreuth, Germanyb |
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| Abstract: | In contrast to orthoretroviruses, processing of foamy viral p71 Gag is limited to a single cleavage site. Nevertheless, Gag maturation is essential for infectivity, but deletion of p3 results in a modest drop in infectivity. Here, we show that Gag processing of p71 to p68 and p3 is essential for full-length cDNA synthesis, while inactivation of Gag cleavage results in cDNAs containing only the RU5 region; cDNAs encompassing the U3 region were almost undetectable. |
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