Silicon Dioxide Thin Film Mediated Single Cell Nucleic Acid Isolation |
| |
Authors: | Evgeny Bogdanov Irina Dominova Natalia Shusharina Stepan Botman Vitaliy Kasymov Maksim Patrushev |
| |
Institution: | 1. Immanuel Kant Baltic Federal University, Kaliningrad, Russia.; 2. University College London, London, United Kingdom.; Indian Institute of Science, India, |
| |
Abstract: | A limited amount of DNA extracted from single cells, and the development of single cell diagnostics make it necessary to create a new highly effective method for the single cells nucleic acids isolation. In this paper, we propose the DNA isolation method from biomaterials with limited DNA quantity in sample, and from samples with degradable DNA based on the use of solid-phase adsorbent silicon dioxide nanofilm deposited on the inner surface of PCR tube. |
| |
Keywords: | |
|
|