Unravelling the Complexity of Human Olfactory Receptor Repertoire by Copy Number Analysis across Population Using High Resolution Arrays |
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| Authors: | Avinash M Veerappa Sangeetha Vishweswaraiah Kusuma Lingaiah Megha Murthy Dinesh S Manjegowda Radhika Nayaka Nallur B Ramachandra |
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| Institution: | 1. Genomics Lab, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore, India.; 2. Department of Anatomy, Yenepoya Medical College, Yenepoya University, Mangalore, India.; Institut Jacques Monod, France, |
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| Abstract: | Olfactory receptors (OR), responsible for detection of odor molecules, belong to the largest family of genes and are highly polymorphic in nature having distinct polymorphisms associated with specific regions around the globe. Since there are no reports on the presence of copy number variations in OR repertoire of Indian population, the present investigation in 43 Indians along with 270 HapMap and 31 Tibetan samples was undertaken to study genome variability and evolution. Analysis was performed using Affymetrix Genome-Wide Human SNP Array 6.0 chip, Affymterix CytoScan® High-Density array, HD-CNV, and MAFFT program. We observed a total of 1527 OR genes in 503 CNV events from 81.3% of the study group, which includes 67.6% duplications and 32.4% deletions encompassing more of genes than pseudogenes. We report human genotypic variation in functional OR repertoire size across populations and it was found that the combinatorial effect of both “orthologous obtained from closely related species” and “paralogous derived sequences” provide the complexity to the continuously occurring OR CNVs. |
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