Cryptosporidium hominis Infection Diagnosed by Real-Time PCR-RFLP |
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| Authors: | Hyeng-Il Cheun Kyungjin Kim Sejoung Yoon Won-Ja Lee Woo-Yoon Park Seobo Sim Jae-Ran Yu |
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| Affiliation: | 1.Department of Malaria and Parasitic Diseases, National Institute of Health, Chungcheongbuk-do 363-951, Korea.;2.Division of Epidemic Intelligence Service, Korea Centers for Disease Control & Prevention, Chungcheongbuk-do 363-951, Korea.;3.Department of Environmental and Tropical Medicine, Konkuk University, School of Medicine, Seoul 143-701, Korea.;4.Department of Radiation Oncology, College of Medicine, Chungbuk National University, Cheongju 361-711, Korea. |
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| Abstract: | There are approximately 20 known species of the genus Cryptosporidium, and among these, 8 infect immunocompetent or immunocompromised humans. C. hominis and C. parvum most commonly infect humans. Differentiating between them is important for evaluating potential sources of infection. We report here the development of a simple and accurate real-time PCR-based restriction fragment length polymorphism (RFLP) method to distinguish between C. parvum and C. hominis. Using the CP2 gene as the target, we found that both Cryptosporidium species yielded 224 bp products. In the subsequent RFLP method using TaqI, 2 bands (99 and 125 bp) specific to C. hominis were detected. Using this method, we detected C. hominis infection in 1 of 21 patients with diarrhea, suggesting that this method could facilitate the detection of C. hominis infections. |
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| Keywords: | Cryptosporidium parvum Cryptosporidium hominis real-time PCR RFLP TaqI |
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