Cancer Stem Cell-Like Side Population Cells in Clear Cell Renal Cell Carcinoma Cell Line 769P |
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| Authors: | Bin Huang Yi Jun Huang Zhi Jun Yao Xu Chen Sheng Jie Guo Xiao Peng Mao Dao Hu Wang Jun Xing Chen Shao Peng Qiu |
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| Affiliation: | 1. Department of Urology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China.; 2. Department of Pharmacology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, China.; 3. Department of Urology, Sun Yat-Sen University Cancer Center, Guangzhou, China.; Cedars Sinai Medical Center, United States of America, |
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| Abstract: | Although cancers are widely considered to be maintained by stem cells, the existence of stem cells in renal cell carcinoma (RCC) has seldom been reported, in part due to the lack of unique surface markers. We here identified cancer stem cell-like cells with side population (SP) phenotype in five human RCC cell lines. Flow cytometry analysis revealed that 769P, a human clear cell RCC cell line, contained the largest amount of SP cells as compared with other four cell lines. These 769P SP cells possessed characteristics of proliferation, self-renewal, and differentiation, as well as strong resistance to chemotherapy and radiotherapy that were possibly related to the ABCB1 transporter. In vivo experiments with serial tumor transplantation in mice also showed that 769P SP cells formed tumors in NOD/SCID mice. Taken together, these results indicate that 769P SP cells have the properties of cancer stem cells, which may play important roles in tumorigenesis and therapy-resistance of RCC. |
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