Development and Characterization of a Chemically Defined Food for Drosophila
|
| |
Authors: | Wen-Chih Lee Craig A. Micchelli |
| |
Affiliation: | Department of Developmental Biology, Washington University School of Medicine, St. Louis, Missouri, United States of America.; VIB and KU Leuven, Belgium, |
| |
Abstract: | Diet can affect a spectrum of biological processes ranging from behavior to cellular metabolism. Yet, the precise role of an individual dietary constituent can be a difficult variable to isolate experimentally. A chemically defined food (CDF) permits the systematic evaluation of individual macro- and micronutrients. In addition, CDF facilitates the direct comparison of data obtained independently from different laboratories. Here, we report the development and characterization of a CDF for Drosophila. We show that CDF can support the long-term culture of laboratory strains and demonstrate that this formulation has utility in isolating macronutrient from caloric density requirements in studies of development, longevity and reproduction. |
| |
Keywords: | |
|
|