Involvement of MicroRNAs in Infection of Silkworm with Bombyx mori Cytoplasmic Polyhedrosis Virus (BmCPV) |
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| Authors: | Ping Wu Shaohua Han Tao Chen Guangxing Qin Long Li Xijie Guo |
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| Affiliation: | 1. Sericultural Research Institute, Jiangsu University of Science and Technology, Zhenjiang Jiangsu, China.; 2. Quality Inspection Center for Sericulture Products, Ministry of Agriculture, Zhenjiang Jiangsu, China.; Nanyang Technological University, Singapore, |
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| Abstract: | Bombyx mori cytoplasmic polyhedrosis virus (BmCPV) is one of the most important pathogens of silkworm. MicroRNAs (miRNAs) have been demonstrated to play key roles in regulating host-pathogen interaction. However, there are limited reports on the miRNAs expression profiles during insect pathogen challenges. In this study, four small RNA libraries from BmCPV-infected midgut of silkworm at 72 h post-inoculation and 96 h post-inoculation and their corresponding control midguts were constructed and deep sequenced. A total of 316 known miRNAs (including miRNA*) and 90 novel miRNAs were identified. Fifty-eight miRNAs displayed significant differential expression between the infected and normal midgut (P value < = 0.01 and fold change > = 2.0 or < = 0.5), among which ten differentially expressed miRNA were validated by qRT-PCR method. Further bioinformatics analysis of predicted target genes of differentially expressed miRNAs showed that the miRNA targets were involved in stimulus and immune system process in silkworm. |
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