Identification of an inducible penicillinase of the lithoautotrophic hydrogen-oxidizing bacteriumAlcaligenes eutrophus |
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Authors: | P. Šebo J. Šťastná |
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Affiliation: | (1) Institute of Microbiology, Czechoslovak Academy of Sciences, 14220 Prague 4 |
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Abstract: | The growth ofAlcaligenes eutrophus in the presence of benzylpenicillin under heterotrophic and autotrophic conditions was studied. The drug induced a penicillinase in the cells, which can be readily released and extracted from the cells after a lysozyme and EDTA treatment in the course of spheroplast formation. The isoelectric point of the enzyme is 8.1 and the molar mass was estimated to be nearly 25 kg/mol. Phenoxypenicillin is hydrolyzed in the presence of the enzyme at a higher relative rate then benzylpenicillin, ampicillin, amoxycillin and azlocillin. The cephalosporins tested,i.e. cephalosporin C, cefalexin, cefotaxime and 7-aminocephalosporanic acid, were hydrolyzed at a substantially lower relative rate than the penicillins, indicating that the enzyme is a penicillinase. |
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