Identification of sn-2-omega-hydroxycarboxylate-containing phospholipids in a lipid extract from bovine brain |
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Authors: | A Tokumura T Tanaka T Yotsumoto H Tsukatani |
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Affiliation: | Faculty of Pharmaceutical Sciences, University of Tokushima, Japan. |
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Abstract: | Phospholipids having both a long-chain acyl (palmitoyl or stearoyl) and a short-chain hydroxycarboxylyl (C3-C9) residue were identified by GC-MS in a fraction with PAF-like activity from a bovine brain lipid extract. The hydroxyl group in the hydroxycarboxylate residue was determined to be at the omega-position by comparison of the mass spectra of the tert-butyl-dimethylsilyl derivatives of these compounds with those of synthetic hydroxybutyrate-containing phosphatidylcholines. The co-existence of short-chain hydroxycarboxylate-, monocarboxylate- and dicarboxylate-containing phospholipids in the bovine brain lipid extract suggested that these compounds were formed by peroxidation of membrane phospholipids, especially phosphatidylcholines. |
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