Carbon catabolite regulation of cephamycin C and expandase biosynthesis in Streptomyces clavuligerus |
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Authors: | Ahmed Lebrihi Gerard Lefebvre Pierre Germain |
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Affiliation: | (1) Laboratoire de Microbiologie Industrielle, ENSAIA, Institut National Polytechnique de Lorraine, Nancy, France;(2) Avenue de la Forêt de Haye, 54500 Vandoeuvre-Lès-Nancy, France |
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Abstract: | Summary Streptomyces clavuligerus produces cephamycin C while growing on chemically defined basal medium. Cephamycin C production takes place during the exponential growth phase and is accompanied by vigorous activity of the cephamycin C synthetase system and of expandase. An excessive amount of glycerol decreases cephamycin C production. Its negative effect appears to be greatest when it is added in the first phase of fermentation either alone or in the presence of starch. Starch excess also reduces cephamycin C production, but its effect is slight compared with glycerol. Glycerol hinders cephamycin C production by the repression of the cephamycin C synthetase system and particularly expandase biosynthesis. Starch and glycerol inhibit neither cephamycin C synthetase nor expandase activities. However, the phosphorylated intermediates of the glycolytic pathway, glucose 6-phosphate and fructose 1,6-phosphate, strongly inhibit expandase activity. |
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