Recombination between satellite phage P4 and its helper P2. I. In vivo and in vitro construction of P4: :P2 hybrid satellite phage |
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| Authors: | B H Lindqvist |
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| Institution: | 1. Institute of Medical Biology, University of Tromsø, 9001 Tromsø Norway;2. Department of Molecular Biology, University of California, Berkeley, CA 94720 U.S.A. |
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| Abstract: | P4::P2 hybrid satellite phages which carry a portion (including the P2 head gene Q and the cohesive end) of the left end of the P2 chromosome linked to the essential part of the P4 chromosome have been isolated by in vivo as well as in vitro recombination. These hybrids express gene Q and grow in the presence of a P2 helper even if defective in gene Q. |
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| Keywords: | Recombinant DNA restriction endonucleases bp base pairs EtBr ethidium bromide kb kilobase pairs SDS sodium dodecyl sulfate SSC 0 15 M NaCl 0 015 M Na · citrate pH 7 8 |
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