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Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis |
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| Authors: | Li Na Liu Zhong Quan Wang Xi Zhang Peng Jiang Xin Qi Ruo Dan Liu Zi Fang Zhang Jing Cui |
| Institution: | Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou, Henan, People''s Republic of China, ; National Institute of Parasitic Diseases Chinese Center for Diseases Control and Prevention, CHINA, |
| Abstract: | BackgroundSparganosis is a neglected but important food-borne parasitic zoonosis. Clinical diagnosis of sparganosis is difficult because there are no specific manifestations. ELISA using plerocercoid crude or excretory–secretory (ES) antigens has high sensitivity but has cross-reactions with other helminthiases. The aim of this study was to characterize Spirometra erinaceieuropaei cysteine protease (SeCP) and to evaluate its potential application for serodiagnosis of sparganosis.ConclusionsThe rSeCP had the CP enzymatic activity and SeCP seems to be important for the survival of plerocercoids in host. The rSeCP is a potential diagnostic antigen for sparganosis. |
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