L-天冬酰胺酶与产物的相互作用

本文从几方面探讨了产物L-天冬氨酸和L-天冬酰胺酶之间的相互作用。产物与珀琥酸对酶荧光影响的比较研究以及产物存在下对碘离子淬灭酶荧光性质的观察表明产物和酶之间存在直接的相互作用。产物保护天冬酰胺酶抗胰蛋白酶水解及抗热失活作用暗示它结合在酶的活性部位附近。

THE STUDY ON THE INTERACTION BETWEEN L-ASPARAGINASE AND THE PRODUCT

The present studies have shown that there are direct interactions between L-as-partic acid as a product of the enzyme-catalyzed hydrolysis of L-asparagine and L-asparaginase by the comparative studies on the effects of the product and succinic acid on the enzyme fluorescence and by the observation of the quenching of the enzyme fluorescence with iodine ions when the product is present in the enzyme solution.L-aspartic acid is able to protect the enzyme from inactivation by proteases and thermal treatment, which suggests that L-aspartic acid combines with the enzyme after all of the substrate molecules are turned into the product molecules and locates somewhere nearby the active center of the enzyme.