A coupled optical assay for determination of adenine in mixtures. |
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| Authors: | U Mura M Romano F Sgarrella P L Ipata |
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| Institution: | Laboratory of Biochemistry, Faculty of Science, University of Pisa, via A, Volta 4, 56100 Pisa, Italy |
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| Abstract: | A rapid and specific spectrophotometric assay for the determination of adenine is described. The method is based on the absorbance change at 265 nm which accompanies the ribose 1-phosphate-dependent conversion of adenine into inosine, catalyzed by the successive action of adenosine phosphorylase and adenosine deaminase. Common purine and pyrimidine bases, nucleosides, and nucleotides do not interfere. The assay was tested in various biochemical situations, in which there was both adenine formation and utilization. |
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