激素对贯叶连翘器官分化的影响

贯叶连翘 (ＨｙｐｅｒｉｃｕｍｐｅｒｆｏｒａｔｕｍＬ .)为多年生草本 ,中国民间主要用于止血、抗炎、妇科病等[1] ,欧洲民间用于治疗创伤也有相当长的历史。近年来 ,欧、美等国家和地区将其应用于抑郁症的治疗 ,取得了很好的疗效。 80年代后期 ,由于发现该植物体内含有显著抗

The influence of phytohormone on organogenesis of Hypericum perforatum L.

Comparative experiments of the induction of buds and roots from the explants of cotyledon, hypocotyl and young leaves of %Hypericum perforatum %L. under the different kinds and concentrations of hormones were carried out %in vitro. %The results indicated that bud differentiation of explants on MS medium with BA and NAA could be observed, and the ratio of BA to NAA influenced the organogenesis of explants to a great extent. When the concentration of BA was 3 mg/L, the optimal concentration of NAA for bud differentiation of cotyledon, hypocotyl and young leaves were 0.2 mg/L, 0.1 mg/L, 1.0 mg/L respectively. Rooting was observed when NAA 1.0 mg/L or the combination of NAA 0.5 mg/L with IBA 0.5 mg/L supplemented to the MS medium, and eventually grew into a complete plantlet.