PCR-RFLP analysis of the Cryptosporidium oocyst wall protein (COWP) gene discriminates between C. wrairi and C. parvum, and between C. parvum isolates of human and animal origin |
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| Authors: | Furio Spano Lorenza Putignani James McLauchlin David P Casemore rea Crisanti |
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| Affiliation: | Istituto di Parassitologia, Universitàdi Roma `La Sapienza', Ple. A. Moro 5, 00185 Rome, Italy;Food Hygiene Laboratory, Public Health Laboratory Service, Central Public Health Laboratory, 61 Colindale Avenue, London NW9 5HT, UK;Public Health Laboratory Service, Cryptosporidium Reference Unit, Glan Clwyd District General Hospital, Rhyl, Clwyd LL18 5UJ, UK;Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB, UK |
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| Abstract: | Cryptosporidium wrairi was isolated from guinea pigs during a spontaneous outbreak of cryptosporidiosis. Despite the morphological and antigenic similarities to C. parvum, C. wrairi displayed a different host range and site of infection and may represent a separate species or sub-species. We used the polymerase chain reaction to clone two distinct 550 bp-long DNA fragments, Wc-I and Wc-II, of the gene encoding the Cryptosporidium oocyst wall protein (COWP) of C. wrairi, which showed 98% identity to the C. parvum homologue. Within Wc-I, polymorphic RsaI restriction sites were used to develop a polymerase chain reaction-restriction fragment length polymorphism method able to distinguish C. wrairi from C. parvum and to identify two groups of C. parvum isolates differentially associated with animal and human infections. |
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| Keywords: | Cryptosporidium wrairi Cryptosporidium parvum Cryptosporidium oocyst wall protein gene Genetic marker Polymerase chain reaction |
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